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The maintenance of chromatographic column and use standard
From;    Author:Stand originally

The maintenance of chromatographic column
1, use protection column
2, the PH stable range of most column is 2-7.5, use inside this limits as far as possible
3, avoid composition of photograph of going from place to place and polarity to have acuteness change
4, before photograph of going from place to place is used, must be enraged via taking off and filter processing
5, if use the amortize solution of polarity or ionic sex,make appearance of going from place to place, want to end to rinse post clean in the experiment
6, protective column changes when pressure is elevatory

The fine be convenient to use of HPLC chromatographic column uses a standard
Before 1. is used every time, the film that uses 0.45um/0.22um filters photograph of going from place to place
The chromatographic column that 2. carries the desiccate in saving a process medium or for a long time needs to use methyl alcohol / second nitrile is complete embellish is wet
3. must consider solvent to reach detached influence to the dissolvability of sample when sample is detached
4. should undertake pretreatment according to property to sample
5. handles good PH, temperature, wait for the element influence to post
6. as far as possible use the photograph of going from place to place that matchs now, the attention prevents photograph of going from place to place to grow the undesirable phenomenon such as bacterium to happen
7. periodic uses appropriate dissolvent, likely course undertakes second birth to post
8. stores when chromatographic column, should amortize salt displacement clean, use chromatographic column of saturation of tall proportional organic dissolvent (second nitrile / methanol)
9, avoid outside force to injure chromatographic column: Bend, decline or puissant shake