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The content of glucoside of the root of large-flowere skullcap in laborious Qin
From;    Author:Stand originally

[prescription] 200g of jingjie of 200g of Huang Qin of 200g of the root of Chinese wild ginger
200g of the achene of Siberian coklebur of 200g of windbreak 200g Bai Zhi
200g of cassia twig of 200g of the rhizome of large-headed atractylodes of 200g of the root of remembranous milk vetch
Grass-leafed sweetflag 200g
[make a way] above 10 flavour, add water decoction 2 times, for the first time 1.5 hours, the 2nd 1 hour, decoct fluid filtration, filtrate is amalgamative, condense to right amount, sparge is dry, join right amount complementary makings, make grain, dry, make 1000g, get namely.
[character] taste the yellow that it is palm to come originally the grain with Brown palm, taste is small sweet, small suffering.
[differentiate] (1) take this tasting 10g, grind fine add methanol 30ml, supersonic processing 20 minutes, filtration, filtrate evaporate works, the residual 15ml that use water is deliquescent, use ether brace up shake extract 3 times (20, 15, 15ml) , amalgamative ether draws liquid, brandish works, residual add liquefy of 1ml of ester of acetic acid second, as taste solution for trying. Take the root of Chinese wild ginger additionally to contrast medicinal material 1g, with solution of legal contrast medicinal material. Illuminate B of Ⅵ of appendix of law of layer color atlas) experiment, absorb two kinds of afore-mentioned solution L of μ of each 10, nod respectively at G of same silica gel on layer board, with personal alkyl - ester of acetic acid second - chloroform (1.5 of ∶ of 2 of 8 ∶ ) to develop an agent, spread out, take out, air, gush with 5% herb aldehyde vitriolic solution, carbonado in 105 ℃ to clarity of stain colour developing. In tasting color atlas for trying, in with contrast medicinal material color atlas is corresponding locally, show same color advocate stain.
(2) take this tasting 1.5g, grind fine, add alcohol 20ml, supersonic processing 10 minutes, filtration, filtrate evaporate works, residual add methanol 1ml liquefy, as taste solution for trying. Take glucoside of the root of large-flowere skullcap additionally to contrast article, add methanol to make the solution that contains 1mg into every 1ml, as contrast taste solution. Take way of layer color atlas (appendix Ⅵ B) experiment, absorb two kinds of afore-mentioned solution L of μ of each 10, nod respectively at same the carboxyl of natrium of acetic acid of the % that contain 4 is methylic the silica gel G that cellulose natrium solution is bind agent on layer board, with ester of acetic acid second - fourth ketone - formic acid - water (1 of ∶ of 1 of ∶ of 3 of 5 ∶ ) to develop an agent, spread out, take out, air, gush with solution of alcohol of iron of 1% 3 chloridize. In tasting color atlas for trying, in with contrast taste color atlas corresponding locally, show the spot of same color.
(3) take this tasting 10g, grind fine, add alcohol 30ml, supersonic processing 30 minutes, filtration, filtrate brandish works, residual add chloroform 1ml liquefy, as taste solution for trying. Take Bai Zhi additionally to contrast medicinal material 1g, with solution of legal contrast medicinal material. Take way of layer color atlas (appendix Ⅵ B) experiment, absorb two kinds of afore-mentioned solution L of each 10 μ , nod respectively at G of same silica gel on layer board, it is with ether develop an agent, spread out, take out, air, buy ultraviolet light (365nm) next postmortem. In tasting color atlas for trying, in with contrast medicinal material color atlas is corresponding locally, show the fluorescent spot of same color.
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